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Multiple Choice
Which of the following staining procedures employs both a primary dye and a counterstain?
A
Acid-fast staining
B
Gram staining
C
Simple staining
D
Negative staining
Verified step by step guidance
1
Understand the purpose of staining in microbiology: Staining is used to enhance the contrast of microscopic organisms, making them more visible under a microscope.
Learn about the Gram staining procedure: Gram staining is a differential staining technique that uses two dyes to classify bacteria into two groups based on the characteristics of their cell walls.
Identify the primary dye in Gram staining: The primary dye used in Gram staining is crystal violet, which initially stains all cells.
Recognize the role of the counterstain: After the application of a mordant (iodine) and decolorization with alcohol, a counterstain, usually safranin, is applied. This stains the cells that have lost the primary dye, allowing differentiation between Gram-positive and Gram-negative bacteria.
Compare with other staining methods: Acid-fast staining also uses a primary dye and a counterstain, but it is specific for identifying acid-fast bacteria. Simple staining uses only one dye, and negative staining involves staining the background instead of the organism.