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Multiple Choice
Oil immersion microscopy typically uses which objective lens for optimal resolution?
A
100x objective lens
B
4x objective lens
C
10x objective lens
D
40x objective lens
Verified step by step guidance
1
Understand the purpose of oil immersion in microscopy: Oil immersion is used to increase the resolution of a microscope by reducing the refraction of light. This is particularly useful for observing fine details in specimens.
Identify the typical magnification range for oil immersion lenses: Oil immersion is generally used with high magnification lenses, typically 100x, to achieve the best resolution.
Consider the options provided: 4x, 10x, 40x, and 100x objective lenses. Recall that lower magnification lenses (4x, 10x, 40x) do not require oil immersion as they do not provide the level of detail that benefits from reduced light refraction.
Recognize that the 100x objective lens is specifically designed for use with oil immersion, as it allows for the highest level of detail and resolution in the specimen being observed.
Conclude that the 100x objective lens is the optimal choice for oil immersion microscopy, as it maximizes the resolving power of the microscope.