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Multiple Choice
What must be done before viewing a slide at 100x magnification using a compound light microscope?
A
Apply immersion oil to the slide.
B
Switch to a lower magnification objective.
C
Clean the eyepiece lens.
D
Adjust the coarse focus knob.
Verified step by step guidance
1
Begin by ensuring that the slide is properly prepared and placed on the stage of the microscope. This involves securing the slide with stage clips to prevent movement during observation.
Start with the lowest magnification objective lens, typically 4x or 10x, to locate the specimen on the slide. This makes it easier to find and center the area of interest before increasing magnification.
Use the coarse focus knob to bring the specimen into general focus. This knob moves the stage up and down more significantly, allowing for quick adjustments at lower magnifications.
Once the specimen is centered and roughly focused, switch to a higher magnification objective lens, such as 40x, to refine the focus using the fine focus knob. This knob allows for more precise adjustments.
Before switching to the 100x magnification, apply a drop of immersion oil directly onto the slide where the light will pass through. This oil reduces light refraction and improves image clarity at high magnifications. Then, carefully switch to the 100x oil immersion objective lens and use the fine focus knob to achieve a clear image.