DRAW IT Copy the polynucleotide strand in Figure 5.23a and label the bases G, T, C, and T, starting from the 5′ end. Assuming this is a DNA polynucleotide, now draw the complementary strand, using the same symbols for phosphates (circles), sugars (pentagons), and bases. Label the bases. Draw arrows showing the 5'→3' direction of each strand. Use the arrows to make sure the second strand is antiparallel to the first. Hint: After you draw the first strand vertically, turn the paper upside down; it is easier to draw the second strand from the 5′ toward the 3′ direction as you go from top to bottom.
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Start by drawing the first polynucleotide strand vertically, with the 5' end at the top and the 3' end at the bottom.
Label the bases on this strand as G, T, C, and T, starting from the 5' end.
Draw the complementary strand next to the first strand. Remember that in DNA, adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G).
Ensure that the complementary strand is antiparallel to the first strand. This means the 5' end of the complementary strand should be at the bottom, and the 3' end should be at the top.
Draw arrows to indicate the 5' to 3' direction for each strand, ensuring the strands are antiparallel.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
DNA Structure
DNA is composed of two polynucleotide strands that form a double helix. Each strand consists of a sugar-phosphate backbone and nitrogenous bases (adenine, thymine, cytosine, and guanine) that pair specifically (A with T and C with G). Understanding this structure is crucial for accurately drawing and labeling DNA strands.
The two strands of DNA run in opposite directions, known as antiparallel orientation. One strand runs from the 5' to 3' direction, while the complementary strand runs from 3' to 5'. This orientation is essential for the proper pairing of bases and for the functioning of DNA during processes like replication and transcription.
Base pairing rules dictate that adenine pairs with thymine (A-T) and cytosine pairs with guanine (C-G) in DNA. These rules are fundamental for constructing the complementary strand accurately. When drawing the complementary strand, it is important to ensure that the correct bases are paired according to these rules to maintain the integrity of the genetic information.