Cells and Infection

Introduction

Understanding the cellular basis of infection is essential in Anatomy & Physiology. Microorganisms such as bacteria, viruses, fungi, protozoa, helminths, and arthropods can invade the human body, leading to various infectious diseases. This guide summarizes the classification, structure, and pathogenic mechanisms of these microbes.

Bacteria

General Characteristics

• Prokaryotic, single-celled organisms: Bacteria lack a defined nucleus and membrane-bound organelles.

• Reproduction: Asexual, primarily by binary fission.

• Classification: Based on shape, staining properties, motility, and metabolism.

Classification by Shape

Bacteria are classified according to their morphology:

• Cocci: Spherical-shaped bacteria (e.g., Streptococcus, Staphylococcus).

• Bacilli: Rod-shaped bacteria (e.g., Escherichia coli, Pseudomonas).

• Spiral bacteria: Includes vibrio, spirillum, and spirochete forms.

Bacterial Classification Table

Histological Staining Properties

• Gram-positive bacteria: Stain violet due to thick peptidoglycan cell wall.

• Gram-negative bacteria: Stain pink due to thinner peptidoglycan and outer membrane.

• Clinical relevance: Gram staining helps determine antibiotic resistance and guides treatment.

Motility and Metabolism

• Motility: Some bacteria can move within tissues using flagella or other structures.

• Metabolism: Bacteria may be aerobic (require oxygen) or anaerobic (do not require oxygen).

Binary Fission

Binary fission is the primary method of bacterial reproduction. The process involves:

1. Cell elongation and DNA replication.

2. Cell wall and plasma membrane begin to divide.

3. Cross-wall forms completely around divided DNA.

4. Cells separate, resulting in two identical daughter cells.

Example: Bacillus licheniformis divides by binary fission, as shown in microscopy images.

Fungi

General Characteristics

• Eukaryotic cells: Have a defined nucleus and organelles.

• Reproduction: Can be asexual (by division or budding) or sexual (spores, genetic exchange).

• Infection (Mycosis): Fungal infections are called mycoses and can be superficial, subcutaneous, or systemic.

Types of Mycoses

• Superficial: Affect epidermis, nails, hair (e.g., Candida albicans).

• Subcutaneous: Involve deep tissue via skin lesions.

• Systemic: Spread through inhalation or lymphatics.

Viruses

General Characteristics

• Structure: Simpler than prokaryotes; consist of nucleic acid (DNA or RNA) within a protein capsid.

• Replication: Non-living; require a host cell to replicate and reproduce.

• Classification: Based on nucleic acid type (single or double-stranded DNA/RNA) and encapsulation (naked or enveloped).

Virus Classification Table

Protozoa, Helminths, and Arthropods

Protozoa

• Single-celled eukaryotes: Reproduce asexually, often using host for part of their life cycle.

• Examples: Amoeba, Giardia, Cryptosporidium.

Helminths

• Multicellular organisms: Have organ systems; feed off host nutrients.

• Examples: Tapeworms (Taenia solium), hookworms (Ancylostoma duodenale), roundworms (Ascaris lumbricoides).

Helminth Infection Table

Arthropods

• Multicellular organisms: Include lice, mites, ticks, maggots; anchor and feed off host.

Colonisation and Pathogenicity

Normal Flora and Symbiosis

• Colonisation: Microorganisms inhabit the body as normal flora.

• Symbiotic relationship: Mutually beneficial; e.g., gut flora obtain nutrients and compete with pathogens.

• Opportunistic infections: Occur when symbiotic microbes breach barriers or host defenses are compromised.

Pathogenicity and Virulence

• Pathogenicity: Capacity of a microbe to cause disease.

• Virulence: Ability to disable host defense systems.

• Exotoxins: Released by organisms, cause host disease.

• Endotoxins: Part of organism's cell membrane, can trigger host responses.

Factors Affecting Virulence

• Entry into body: Via skin lesions, mucosal surfaces, or other routes.

• Invasiveness: Ability to invade tissues and evade immune responses.

Breaking the Chain of Transmission

Principles

• Interrupting transmission of infectious agents is key to infection control.

• Methods include hygiene, barrier protection, and treatment of carriers.

Summary Table: Microbial Groups

Key Equations

Bacterial Growth Rate

The rate of bacterial population growth can be described by:

• N: Final number of bacteria

• N_0: Initial number of bacteria

• n: Number of generations

Summary

Understanding the classification, structure, and pathogenic mechanisms of microbes is fundamental for studying infection in Anatomy & Physiology. Recognizing how microbes reproduce, invade, and interact with the host informs strategies for prevention and treatment of infectious diseases.