Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

3. Amino Acids

Amino Acid Groups

Biochemistry

3. Amino Acids

Amino Acid Groups

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Multiple Choice

Which tryptophan (Trp) residue of carbonic anhydrase can be selectively modified with chloroform (CHCl$_3$) at 20$^{\circ}$C?

Trp-207

Trp-5

Trp-17

Trp-245

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Verified step by step guidance

Understand the context: Carbonic anhydrase is an enzyme that contains tryptophan (Trp) residues. The question asks which specific Trp residue can be selectively modified with chloroform (CHCl$_3$) at 20°C. This requires knowledge of the enzyme's structure and the chemical reactivity of Trp residues.

Review the chemical properties of tryptophan: Tryptophan is an aromatic amino acid with an indole side chain. Its reactivity depends on its environment within the protein structure, such as accessibility to solvents and proximity to reactive groups.

Consider the selective modification process: Chloroform (CHCl$_3$) can react with accessible Trp residues under specific conditions. The residue's position in the protein structure determines its accessibility and likelihood of modification.

Analyze the structural environment of each Trp residue: Trp-5, Trp-17, Trp-207, and Trp-245 are potential candidates. Use structural data (e.g., from X-ray crystallography or molecular modeling) to determine which residue is most exposed to the solvent and reactive under the given conditions.

Conclude based on structural and chemical analysis: Based on the enzyme's structure and the reactivity of Trp residues, identify the residue that is most likely to be selectively modified by chloroform at 20°C. This residue is Trp-207, as it is likely the most accessible and reactive under these conditions.