Multiple Choice
Which of the following best describes the role of enzymes in biochemical reactions?
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6. Enzymes and Enzyme Kinetics
Enzymes
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Which of the following enzymes is commonly used to synthesize radioactively labeled DNA in vitro?
A
RNA polymerase
B
DNA polymerase I
C
Ligase
D
Reverse transcriptase
Verified step by step guidance1
Understand the role of each enzyme listed in the problem. RNA polymerase synthesizes RNA from a DNA template, ligase joins DNA fragments, reverse transcriptase synthesizes DNA from an RNA template, and DNA polymerase I synthesizes DNA from a DNA template.
Recall that radioactively labeled DNA is synthesized in vitro by incorporating radioactive nucleotides into the DNA strand during replication.
Identify the enzyme responsible for DNA synthesis. DNA polymerase I is specifically involved in synthesizing DNA by adding nucleotides to a DNA strand using a DNA template.
Consider why DNA polymerase I is suitable for this process. It has the ability to incorporate modified nucleotides, such as radioactively labeled ones, into the growing DNA strand.
Conclude that DNA polymerase I is the correct enzyme for synthesizing radioactively labeled DNA in vitro, as it directly facilitates the incorporation of labeled nucleotides during DNA replication.
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