Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

6. Enzymes and Enzyme Kinetics

Enzymes

Biochemistry

6. Enzymes and Enzyme Kinetics

Enzymes

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Multiple Choice

Which enzyme, isolated from retroviruses, is commonly used to generate complementary DNA (cDNA) from an RNA template?

Reverse transcriptase

Ligase

DNA polymerase I

RNA polymerase

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Verified step by step guidance

Step 1: Understand the role of the enzyme in question. The problem asks for an enzyme that generates complementary DNA (cDNA) from an RNA template. This process is known as reverse transcription.

Step 2: Recall the function of reverse transcriptase. Reverse transcriptase is an enzyme commonly isolated from retroviruses, and its primary function is to synthesize cDNA from an RNA template during the replication of retroviral genomes.

Step 3: Compare the functions of the other enzymes listed. Ligase is involved in joining DNA fragments, DNA polymerase I is responsible for DNA synthesis and repair, and RNA polymerase synthesizes RNA from a DNA template. None of these enzymes are involved in reverse transcription.

Step 4: Recognize that reverse transcriptase is unique in its ability to perform reverse transcription, making it the correct enzyme for generating cDNA from RNA.

Step 5: Conclude that the correct answer is reverse transcriptase, as it matches the description provided in the problem and is commonly used in molecular biology for cDNA synthesis.