Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

6. Enzymes and Enzyme Kinetics

Enzyme Kinetics

Biochemistry

6. Enzymes and Enzyme Kinetics

Enzyme Kinetics

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Multiple Choice

Given the following data for an enzyme-catalyzed reaction:| [S] (mM) | Initial velocity ($v_0$) (μmol/min) ||----------|-----------------------------------|| 0.5 | 2.0 || 1.0 | 3.3 || 2.0 | 4.4 || 5.0 | 5.0 |What is the approximate $V_{max}$ for this enzyme under these conditions?

10.0 μmol/min

3.3 μmol/min

2.0 μmol/min

5.0 μmol/min

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Verified step by step guidance

Step 1: Understand the concept of $V_{max}$. In enzyme kinetics, $V_{max}$ represents the maximum velocity of the reaction when the enzyme is saturated with substrate. It is the plateau of the Michaelis-Menten curve where increasing substrate concentration no longer increases the reaction rate.

Step 2: Analyze the data provided in the table. Look at the relationship between substrate concentration ([S]) and initial velocity ($v_0$). Notice that as [S] increases, $v_0$ approaches a maximum value.

Step 3: Identify the highest initial velocity ($v_0$) in the table. This value corresponds to the approximate $V_{max}$ because it represents the point where further increases in [S] do not significantly increase the reaction rate.

Step 4: Compare the given answer choices to the highest $v_0$ value in the table. The correct $V_{max}$ should match the highest observed velocity.

Step 5: Conclude that under these experimental conditions, the approximate $V_{max}$ is the highest $v_0$ value observed in the table, which is 5.0 μmol/min.