Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

6. Enzymes and Enzyme Kinetics

Enzyme Kinetics

Biochemistry

6. Enzymes and Enzyme Kinetics

Enzyme Kinetics

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Multiple Choice

Which of the following methods is commonly used to determine enzyme activity levels in a laboratory setting?

Determining the amino acid sequence of the enzyme by mass spectrometry

Observing changes in enzyme tertiary structure using X-ray crystallography

Measuring the rate of product formation over time using a spectrophotometric assay

Measuring the enzyme's molecular weight using gel electrophoresis

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Verified step by step guidance

Step 1: Understand the concept of enzyme activity. Enzyme activity refers to the ability of an enzyme to catalyze a chemical reaction. It is typically measured by monitoring the rate at which a substrate is converted into a product.

Step 2: Recognize the most common method for measuring enzyme activity. Spectrophotometric assays are widely used because they allow researchers to monitor changes in absorbance or fluorescence over time, which correlates with the formation of the product.

Step 3: Learn how spectrophotometric assays work. In these assays, the reaction mixture contains the enzyme, substrate, and any necessary cofactors. As the enzyme catalyzes the reaction, the product either absorbs light at a specific wavelength or fluoresces, and this change is measured using a spectrophotometer.

Step 4: Compare the other methods listed in the problem. Determining the amino acid sequence by mass spectrometry, observing tertiary structure changes using X-ray crystallography, and measuring molecular weight using gel electrophoresis are techniques used for structural or compositional analysis, not for measuring enzyme activity.

Step 5: Conclude that measuring the rate of product formation over time using a spectrophotometric assay is the correct method for determining enzyme activity levels in a laboratory setting.