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Multiple Choice
In the context of nucleic acids, what is a restriction enzyme?
A
A DNA ligase that joins DNA fragments by forming phosphodiester bonds
B
A bacterial endonuclease that recognizes specific DNA sequences and cleaves the DNA at or near those sites
C
A helicase that unwinds double-stranded DNA by breaking hydrogen bonds between base pairs
D
An RNA polymerase that synthesizes RNA from a DNA template during transcription
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Verified step by step guidance
1
Understand that the question asks for the definition of a restriction enzyme in the context of nucleic acids.
Recall that restriction enzymes are proteins that interact specifically with DNA molecules, particularly recognizing certain sequences.
Identify that restriction enzymes are bacterial endonucleases, meaning they cut DNA internally rather than chewing from the ends.
Note that these enzymes recognize specific palindromic DNA sequences and cleave the DNA at or near these recognition sites, which is crucial for molecular cloning and genetic analysis.
Distinguish restriction enzymes from other enzymes listed, such as DNA ligase (which joins DNA fragments), helicase (which unwinds DNA), and RNA polymerase (which synthesizes RNA), to confirm the correct definition.