Table of contents

1. Introduction to Biochemistry4h 34m
2. Water3h 23m
3. Amino Acids8h 10m
4. Protein Structure10h 4m
5. Protein Techniques14h 5m
6. Enzymes and Enzyme Kinetics13h 38m
7. Enzyme Inhibition and Regulation 8h 42m
8. Protein Function 9h 41m
9. Carbohydrates7h 49m
10. Lipids5h 49m
11. Biological Membranes and Transport 6h 37m
12. Biosignaling9h 45m
Review 1: Nucleic Acids, Lipids, & Membranes2h 47m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway3h 12m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism2h 26m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation1h 48m

6. Enzymes and Enzyme Kinetics

Lineweaver-Burk Plot

Biochemistry

6. Enzymes and Enzyme Kinetics

Lineweaver-Burk Plot

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Multiple Choice

Consider the equation for the line on the following Lineweaver-Burk plot: y = 6x + 3

A) What is the K_m for the corresponding enzyme?
a) -2 mM
b) 18 mM
c) 2 mM
d) 9 mM
B) What is the V_max for the corresponding enzyme?
a) 0.333 mM/s
b) 9 mM/s
c) -6 mM/s
d) 18 mM/s

-2 mM

18 mM

2 mM

9 mM

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Verified step by step guidance

The Lineweaver-Burk plot is a double reciprocal plot used to illustrate enzyme kinetics. The equation of the line is given as y = 6x + 3, which is in the form of y = mx + c, where m is the slope and c is the y-intercept.

In a Lineweaver-Burk plot, the y-intercept (c) is equal to 1/Vmax. From the equation y = 6x + 3, the y-intercept is 3. Therefore, 1/Vmax = 3.

To find Vmax, take the reciprocal of the y-intercept: Vmax = 1/3. This will help you determine the correct answer for part B.

The slope (m) of the Lineweaver-Burk plot is equal to Km/Vmax. From the equation y = 6x + 3, the slope is 6. Therefore, Km/Vmax = 6.

To find Km, use the relationship Km = slope * Vmax. Substitute the values you have for the slope and Vmax to calculate Km, which will help you determine the correct answer for part A.