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Multiple Choice
What is the role of the tracrRNA in the CRISPR-Cas9 genome defense system?
A
It guides the Cas9 enzyme to the target DNA sequence.
B
It repairs DNA breaks introduced by the Cas9 enzyme.
C
It acts as a template for synthesizing new RNA molecules.
D
It degrades foreign RNA molecules in the cell.
Verified step by step guidance
1
Understand the CRISPR-Cas9 system: It is a bacterial immune system that provides defense against foreign genetic elements, such as plasmids and phages.
Identify the components of the CRISPR-Cas9 system: The main components include the Cas9 enzyme, guide RNA (gRNA), and tracrRNA.
Learn the function of tracrRNA: In the CRISPR-Cas9 system, tracrRNA is a small RNA molecule that is essential for the maturation of the guide RNA (gRNA) and for guiding the Cas9 enzyme to the target DNA sequence.
Explore the interaction between tracrRNA and Cas9: The tracrRNA forms a complex with the gRNA, which then binds to the Cas9 enzyme, enabling it to locate and bind to the specific DNA sequence that needs to be cut.
Clarify the role of tracrRNA: Its primary role is to guide the Cas9 enzyme to the target DNA sequence, ensuring precise cutting of the DNA at the desired location.