Examine the role of carbon fixation: In the first step of the Calvin cycle, an enzyme called RuBisCO catalyzes the reaction between carbon dioxide ($\mathrm{CO_2}$) and ribulose-1,5-bisphosphate (RuBP), forming two molecules of 3-phosphoglycerate (3-PGA). This is the step where carbon atoms from $\mathrm{CO_2}$ are integrated into the cycle.