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Multiple Choice
Which of the following enzymes is required to synthesize complementary DNA (cDNA) from RNA?
A
Ligase
B
DNA polymerase
C
Reverse transcriptase
D
RNA polymerase
Verified step by step guidance
1
Understand the process of synthesizing complementary DNA (cDNA) from RNA, which is known as reverse transcription.
Identify the enzyme responsible for reverse transcription. This enzyme is called reverse transcriptase.
Reverse transcriptase catalyzes the formation of cDNA from an RNA template, a process crucial in retroviruses and molecular biology techniques.
Differentiate reverse transcriptase from other enzymes listed: Ligase joins DNA fragments, DNA polymerase synthesizes DNA from a DNA template, and RNA polymerase synthesizes RNA from a DNA template.
Recognize that reverse transcriptase is unique in its ability to use RNA as a template to synthesize DNA, making it essential for cDNA synthesis.