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Multiple Choice
Which technique was crucial for scientists to visualize the structure of DNA and led to the discovery of its double helix?
A
Gel electrophoresis
B
X-ray crystallography
C
Polymerase chain reaction (PCR)
D
Electron microscopy
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Verified step by step guidance
1
Understand the problem: The question asks which technique was crucial for visualizing the structure of DNA and led to the discovery of its double helix. This requires knowledge of the historical methods used in molecular biology.
Review the options: The techniques listed are Gel electrophoresis, X-ray crystallography, Polymerase chain reaction (PCR), and Electron microscopy. Each of these has specific applications in biology.
Analyze the techniques: Gel electrophoresis is used to separate DNA fragments by size, not for visualizing molecular structures. PCR is used to amplify DNA, not for structural visualization. Electron microscopy provides high-resolution images but is not suitable for resolving the atomic structure of DNA.
Focus on X-ray crystallography: This technique involves directing X-rays at a crystallized molecule and analyzing the diffraction pattern to determine the molecule's 3D structure. Rosalind Franklin's X-ray diffraction images of DNA were critical in revealing the double helix structure.
Conclude: Based on the analysis, X-ray crystallography is the correct technique that allowed scientists to visualize the structure of DNA and led to the discovery of its double helix.