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Multiple Choice
Which of the following is a reason to use membrane filtration as a viable cell count method?
A
It allows for the separation of cells based on size.
B
It is faster than direct microscopic counts.
C
It requires no specialized equipment.
D
It can be used to count viruses directly.
Verified step by step guidance
1
Understand the purpose of membrane filtration: Membrane filtration is a technique used to separate and count cells based on their size by passing a liquid sample through a filter with pores of a specific size.
Consider the advantages of membrane filtration: One of the main reasons to use this method is its ability to separate cells based on size, which allows for the counting of viable cells that are retained on the filter.
Evaluate the speed of the method: While membrane filtration is effective, it is not necessarily faster than direct microscopic counts, as it involves additional steps such as filtration and incubation.
Assess the equipment requirements: Membrane filtration does require some specialized equipment, such as a vacuum pump and filtration apparatus, making the statement about no specialized equipment incorrect.
Determine the applicability to viruses: Membrane filtration is generally not used to count viruses directly, as viruses are much smaller than the typical pore sizes used for filtering cells.