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Multiple Choice
Why is only a fraction of an organism's genes represented in any cDNA library?
A
Because cDNA libraries are derived from mRNA, which only includes expressed genes.
B
Because cDNA libraries are generated from tRNA, which only includes transfer genes.
C
Because cDNA libraries are created from DNA, which includes all genes.
D
Because cDNA libraries are synthesized from rRNA, which only includes ribosomal genes.
Verified step by step guidance
1
Understand that a cDNA library is a collection of complementary DNA (cDNA) sequences derived from mRNA molecules present in a cell.
Recognize that mRNA is transcribed from DNA and represents only the genes that are actively expressed in the cell at a given time.
Note that not all genes in an organism's genome are expressed at all times; some genes are only expressed under certain conditions or in specific cell types.
Realize that because cDNA is synthesized from mRNA, a cDNA library will only contain sequences from genes that were being transcribed into mRNA at the time the library was created.
Conclude that a cDNA library represents only a fraction of an organism's genes, specifically those that are actively expressed, rather than the entire genome.