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Multiple Choice
What is the primary function of DNA ligase during DNA repair?
A
It joins breaks in the sugar-phosphate backbone by forming phosphodiester bonds.
B
It removes damaged bases from the DNA strand.
C
It synthesizes new DNA strands using a template.
D
It unwinds the DNA double helix to allow repair enzymes access.
Verified step by step guidance
1
Understand the role of DNA ligase in the context of DNA repair. DNA ligase is an enzyme that facilitates the joining of DNA strands by forming phosphodiester bonds between the sugar-phosphate backbone of nucleotides.
Recognize that DNA ligase is specifically involved in sealing nicks or breaks in the DNA backbone, which can occur during DNA replication, repair, or recombination.
Eliminate incorrect options by analyzing their functions: Removing damaged bases is the role of enzymes like glycosylases in base excision repair, synthesizing new DNA strands is performed by DNA polymerase, and unwinding the DNA double helix is the function of helicase.
Focus on the correct function of DNA ligase, which is to repair breaks in the sugar-phosphate backbone by catalyzing the formation of phosphodiester bonds.
Conclude that the primary function of DNA ligase during DNA repair is to join breaks in the sugar-phosphate backbone by forming phosphodiester bonds, ensuring the integrity of the DNA molecule.