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Multiple Choice
The tRNA shown in the figure has its 3′ end projecting beyond its 5′ end. Which of the following processes will occur at this 3′ end?
A
The amino acid binds covalently.
B
The excess nucleotides (ACCA) will be cleaved off at the ribosome.
C
The small and large subunits of the ribosome will attach to it.
D
These nucleotides represent the anti-codon.
Verified step by step guidance
1
Identify the structure of the tRNA molecule in the image. The tRNA has a cloverleaf structure with an anticodon loop, a D-loop, a TψC loop, and an acceptor stem where the 3' end is located.
Observe that the 3' end of the tRNA extends beyond the 5' end. This is a characteristic feature of tRNA molecules, where the 3' end typically has the sequence CCA.
Understand the function of the 3' end of the tRNA. This end is crucial for the attachment of an amino acid, which is a key step in protein synthesis.
Recognize that the amino acid is covalently attached to the 3' end of the tRNA by an enzyme called aminoacyl-tRNA synthetase. This process is known as 'charging' the tRNA.
Conclude that the correct process occurring at the 3' end of the tRNA is the covalent binding of an amino acid, which is essential for the tRNA to participate in translation at the ribosome.