By which mechanism would a steroid molecule diffuse into the cell?

Facilitated diffusion across a membrane requires ______________ and moves a solute ______________ its concentration gradient.

a. transport proteins . . . up (against)

b. transport proteins . . . down

c. energy and transport proteins . . . up

d. energy and transport proteins . . . down

Cells lining kidney tubules function in the reabsorption of water from urine. In response to chemical signals, they reversibly insert additional aquaporins into their plasma membranes. In which of these situations would your tubule cells have the most aquaporins: after a long run on a hot day, right after a large meal, or after drinking a large bottle of water? Explain.

Mercury is known to inhibit the permeability of water channels. To help establish that the protein isolated by Agre's group was a water channel, the researchers incubated groups of RNA-injected oocytes (which thus made aquaporin proteins) in four different solutions: plain buffer, low concentration and high concentration of a mercury chloride (HgCl₂) solution, and low concentration of a mercury solution followed by an agent (ME) known to reverse the effects of mercury. The water permeability of the cells was determined by the rate of their osmotic swelling. Interpret the results of this experiment, which are presented in the graph below. Control oocytes not injected with aquaporin RNA were also incubated with buffer and the two concentrations of mercury. Predict what the results of these treatments would be.

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