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Multiple Choice
What is the result of DNA ligase's action during DNA replication?
A
Joining of Okazaki fragments on the lagging strand
B
Unwinding of the DNA double helix
C
Proofreading of newly synthesized DNA
D
Synthesis of RNA primers
Verified step by step guidance
1
Understand the role of DNA ligase in DNA replication. DNA ligase is an enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.
Recognize that during DNA replication, the DNA double helix is unwound, and replication occurs on both the leading and lagging strands.
Identify that on the lagging strand, DNA is synthesized in short segments known as Okazaki fragments due to the antiparallel nature of DNA and the unidirectional activity of DNA polymerase.
Realize that DNA ligase's specific function is to join these Okazaki fragments together to create a continuous DNA strand on the lagging strand.
Conclude that the action of DNA ligase is crucial for completing the synthesis of the lagging strand by sealing the nicks between Okazaki fragments, ensuring the integrity and continuity of the DNA molecule.