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Multiple Choice
What is the function of the enzyme primase during DNA replication?
A
It adds nucleotides to the growing DNA strand in a 5' to 3' direction.
B
It unwinds the DNA double helix to allow replication to occur.
C
It synthesizes a short RNA primer to initiate DNA synthesis.
D
It joins Okazaki fragments on the lagging strand.
Verified step by step guidance
1
Understand the role of primase in DNA replication: Primase is an enzyme that synthesizes a short RNA primer, which is necessary for DNA polymerase to begin DNA synthesis.
Recognize the importance of the RNA primer: DNA polymerase cannot initiate the synthesis of a new DNA strand on its own; it requires a primer to provide a starting point.
Identify where primase acts during replication: Primase synthesizes the RNA primer on both the leading and lagging strands, but it is especially crucial for the lagging strand, where multiple primers are needed.
Distinguish primase from other enzymes: Unlike DNA polymerase, which adds nucleotides to the growing DNA strand, primase specifically creates the RNA primer needed for initiation.
Clarify the sequence of events: Primase synthesizes the RNA primer, which is then extended by DNA polymerase to form the new DNA strand.