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Multiple Choice
The protein that binds to the operator of the lac operon to prevent transcription is encoded by which gene?
A
lac I.
B
lac Y.
C
lac A.
D
lac Z.
Verified step by step guidance
1
Understand the lac operon: The lac operon is a set of genes involved in lactose metabolism in E. coli. It includes structural genes (lacZ, lacY, and lacA) and regulatory elements (promoter, operator, and the lacI gene).
Identify the role of the operator: The operator is a DNA sequence that acts as a binding site for a repressor protein, which can block RNA polymerase from transcribing the downstream genes.
Determine the function of the lacI gene: The lacI gene encodes the lac repressor protein, which binds to the operator to prevent transcription of the lac operon when lactose is not present.
Differentiate between the structural genes: The lacZ gene encodes β-galactosidase, lacY encodes permease, and lacA encodes transacetylase. These genes are involved in lactose metabolism but do not encode the repressor.
Conclude which gene encodes the repressor: Based on the roles of the genes, the lacI gene is responsible for encoding the repressor protein that binds to the operator to prevent transcription.