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Ch. 9 - Cellular Respiration and Fermentation
Freeman - Biological Science 7th Edition
Freeman7th EditionBiological ScienceISBN: 9783584863285Not the one you use?Change textbook
Chapter 9, Problem 8

The researchers who observed that magnetite was produced by bacterial cultures from the deep subsurface carried out a follow-up experiment. These biologists treated some of the cultures with a drug that poisons the enzymes involved in electron transport chains. In cultures where the drug was present, no more magnetite was produced. Does this result support or undermine their hypothesis that the bacteria in the cultures perform cellular respiration? Explain your reasoning.

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Step 1: Begin by understanding the hypothesis. The researchers hypothesize that the bacteria in the cultures perform cellular respiration, which is a process that involves electron transport chains to generate energy.
Step 2: Analyze the experimental setup. The researchers treated some bacterial cultures with a drug that poisons enzymes involved in electron transport chains. This drug would disrupt cellular respiration if the bacteria rely on it.
Step 3: Examine the results. In the cultures treated with the drug, no magnetite was produced. This suggests that the production of magnetite is dependent on the bacteria's ability to perform cellular respiration.
Step 4: Connect the results to the hypothesis. Since the drug disrupted magnetite production by interfering with electron transport chains, this supports the hypothesis that the bacteria perform cellular respiration as part of the process leading to magnetite production.
Step 5: Conclude the reasoning. The experiment demonstrates a direct link between cellular respiration and magnetite production, reinforcing the idea that the bacteria rely on electron transport chains for this function.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Cellular Respiration

Cellular respiration is a metabolic process in which cells convert nutrients into energy, primarily in the form of ATP, while producing waste products. This process typically involves glycolysis, the Krebs cycle, and the electron transport chain. In bacteria, this can occur anaerobically or aerobically, depending on the organism and environmental conditions.
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Electron Transport Chain (ETC)

The electron transport chain is a series of protein complexes located in the inner mitochondrial membrane (or the plasma membrane in bacteria) that facilitate the transfer of electrons derived from nutrients. This process generates a proton gradient that drives ATP synthesis. Inhibition of the ETC can halt cellular respiration, leading to a lack of energy production and, in this case, the cessation of magnetite production.
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Magnetite Production

Magnetite is a magnetic iron oxide produced by certain bacteria as a byproduct of their metabolic processes, often linked to their respiratory activities. The production of magnetite can indicate that the bacteria are engaging in cellular respiration, as it is typically associated with the reduction of iron ions during energy generation. Thus, the absence of magnetite in the presence of an ETC-inhibiting drug suggests a disruption in the bacteria's ability to perform respiration.
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Related Practice
Textbook Question

If you were to expose cells that are undergoing aerobic respiration to a radioactive oxygen isotope in the form of O2, which of the following molecules would you expect to be radiolabeled?

a. Pyruvate

b. Water

c. NADH

d. CO2

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Textbook Question

In step 3 of the citric acid cycle, the enzyme isocitrate dehydrogenase is regulated by NADH. Compare and contrast the regulation of this enzyme with the regulation of phosphofructokinase in glycolysis.

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Textbook Question

Explain the relationship between electron transport and oxidative phosphorylation. How do uncoupling proteins 'uncouple' this relationship in brown adipose tissue?

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Textbook Question
Explain the relationship between electron transport and oxidative phosphorylation. How do uncoupling proteins 'uncouple' this relationship in brown adipose tissue?
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Textbook Question
The researchers who observed that magnetite was produced by bacterial cultures from the deep subsurface carried out a follow-up experiment. These biologists treated some of the cultures with a drug that poisons the enzymes involved in electron transport chains. In cultures where the drug was present, no more magnetite was produced. Does this result support or undermine their hypothesis that the bacteria in the cultures perform cellular respiration? Explain your reasoning.
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Textbook Question

Cyanide (C ≡ N) blocks complex IV of the electron transport chain. Suggest a hypothesis for what happens to the ETC when complex IV stops working. Your hypothesis should explain why cyanide poisoning in humans is fatal.

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