Multiple Choice
In the context of DNA replication, what is the main function of DNA polymerase?
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8. DNA Replication
Overview of DNA Replication
Multiple Choice
In DNA replication, what is the primary role of primase?
A
Synthesizes short RNA primers that provide a free group for DNA polymerase to begin DNA synthesis
B
Unwinds the DNA double helix by breaking hydrogen bonds between base pairs
C
Seals nicks between Okazaki fragments by forming phosphodiester bonds
D
Adds complementary DNA nucleotides to the growing strand in the direction without requiring a primer
Verified step by step guidance1
Understand that DNA replication requires an enzyme to initiate the synthesis of a new DNA strand because DNA polymerase cannot start synthesis on a bare single-stranded DNA template.
Recognize that primase is the enzyme responsible for synthesizing a short RNA primer, which provides a free 3\' hydroxyl (3\' OH) group necessary for DNA polymerase to add nucleotides.
Recall that the RNA primer created by primase is complementary to the DNA template strand and serves as a starting point for DNA polymerase to extend the new DNA strand in the 5\' to 3\' direction.
Differentiate primase's role from other enzymes involved in replication, such as helicase (which unwinds DNA), DNA ligase (which seals nicks), and DNA polymerase (which adds DNA nucleotides).
Summarize that the primary role of primase is to synthesize short RNA primers that provide the free 3\' OH group required for DNA polymerase to begin DNA synthesis.
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