Multiple Choice
In the context of an overview of DNA replication, what is the primary purpose of DNA replication in cells?
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8. DNA Replication
Overview of DNA Replication
Multiple Choice
In the context of DNA replication, what is the primary function of DNA polymerase?
A
It synthesizes a new DNA strand by adding nucleotides to the 3′ end using the template strand and forms phosphodiester bonds.
B
It lays down short RNA primers to provide a free 3′ hydroxyl group for DNA synthesis.
C
It joins Okazaki fragments together by sealing nicks in the sugar-phosphate backbone.
D
It unwinds the DNA double helix ahead of the replication fork by breaking hydrogen bonds.
Verified step by step guidance1
Understand that DNA replication involves multiple enzymes, each with a specific role in copying the DNA accurately.
Identify that DNA polymerase is the enzyme responsible for synthesizing the new DNA strand by adding nucleotides complementary to the template strand.
Recall that DNA polymerase adds nucleotides only to the 3\' end of the growing strand, which means synthesis occurs in the 5\' to 3\' direction.
Recognize that DNA polymerase forms phosphodiester bonds between nucleotides, linking them together to create the sugar-phosphate backbone of the new strand.
Differentiate DNA polymerase from other enzymes mentioned: primase lays down RNA primers, ligase joins Okazaki fragments, and helicase unwinds the DNA helix.
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