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Multiple Choice
What do sticky ends allow in the context of DNA manipulation?
A
They cause the DNA to coil more tightly, making it inaccessible to enzymes.
B
They increase the mutation rate during DNA replication.
C
They prevent DNA fragments from binding to each other.
D
They enable the joining of DNA fragments from different sources through complementary base pairing.
Verified step by step guidance
1
Understand that sticky ends are short, single-stranded overhangs created when certain restriction enzymes cut DNA at specific sequences.
Recognize that these overhangs have unpaired bases that can form hydrogen bonds with complementary sequences on other DNA fragments.
Know that this complementary base pairing allows DNA fragments from different sources to anneal or join together.
Realize that this property is crucial in molecular cloning, where DNA fragments from different organisms can be combined to form recombinant DNA.
Conclude that sticky ends facilitate the precise and efficient joining of DNA fragments by providing complementary sequences that guide the ligation process.