Multiple Choice
In the context of DNA replication, what is an RNA primer?
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8. DNA Replication
Overview of DNA Replication
Multiple Choice
In the overview of DNA replication, what is the primary function of DNA polymerase?
A
Seals nicks between Okazaki fragments by forming phosphodiester bonds
B
Synthesizes a new DNA strand by adding deoxyribonucleotides to the end using a DNA template
C
Lays down short RNA primers to provide a free hydroxyl group for DNA synthesis
D
Unwinds the DNA double helix ahead of the replication fork
Verified step by step guidance1
Understand the role of DNA polymerase in DNA replication: DNA polymerase is an enzyme responsible for synthesizing new DNA strands complementary to the template strand.
Recall that DNA polymerase adds nucleotides only to the 3\' end of a growing DNA strand, meaning it synthesizes DNA in the 5\' to 3\' direction.
Recognize that DNA polymerase requires a primer with a free 3\' hydroxyl group to initiate DNA synthesis; it cannot start synthesis de novo.
Differentiate DNA polymerase's function from other enzymes: for example, DNA ligase seals nicks between Okazaki fragments, primase lays down RNA primers, and helicase unwinds the DNA helix.
Conclude that the primary function of DNA polymerase is to synthesize a new DNA strand by adding deoxyribonucleotides to the 3\' end using the DNA template strand.
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