Multiple Choice
Which enzyme is responsible for joining Okazaki fragments together during DNA replication?
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17. Mutation, Repair, and Recombination
DNA Repair
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In bacteria, which function does DNA polymerase I perform during DNA repair?
A
It unwinds the DNA helix ahead of the replication fork.
B
It ligates DNA fragments together after replication.
C
It synthesizes Okazaki fragments on the lagging strand.
D
It removes RNA primers and fills in the vacant regions with DNA nucleotides.
Verified step by step guidance1
Understand the role of DNA polymerase I in bacterial DNA replication and repair processes.
Recall that during DNA replication, RNA primers are laid down to initiate synthesis of new DNA strands, especially on the lagging strand.
Recognize that DNA polymerase I has a unique function: it removes these RNA primers through its 5' to 3' exonuclease activity.
After removing the RNA primers, DNA polymerase I fills in the resulting gaps with DNA nucleotides using its polymerase activity.
Note that other enzymes perform different functions: DNA helicase unwinds the DNA helix, DNA ligase joins DNA fragments, and DNA polymerase III synthesizes Okazaki fragments.
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