Multiple Choice
In a typical semiconservative DNA replication event, what is the end result of DNA replication?
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8. DNA Replication
Overview of DNA Replication
Multiple Choice
In the overview of DNA replication, what is the primary role of DNA polymerase?
A
It synthesizes a new DNA strand by adding nucleotides to the 3′ end of a primer using the template strand for base pairing.
B
It seals breaks in the sugar-phosphate backbone by forming phosphodiester bonds between Okazaki fragments.
C
It unwinds the parental DNA double helix ahead of the replication fork.
D
It lays down short RNA primers to initiate DNA synthesis on the leading and lagging strands.
Verified step by step guidance1
Understand that DNA replication involves multiple enzymes, each with a specific role in copying the DNA accurately.
Identify that DNA polymerase is the enzyme responsible for synthesizing new DNA strands by adding nucleotides complementary to the template strand.
Recognize that DNA polymerase can only add nucleotides to the 3′ end of an existing strand, which means it requires a primer to start synthesis.
Distinguish DNA polymerase's role from other enzymes: DNA ligase seals breaks between Okazaki fragments, helicase unwinds the DNA helix, and primase lays down RNA primers.
Summarize that the primary role of DNA polymerase is to extend the DNA strand by adding nucleotides to the 3′ end of the primer, using the template strand for accurate base pairing.
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