Multiple Choice
What is the dipeptide encoded by the DNA sequence ATGACC?
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7. DNA and Chromosome Structure
DNA as the Genetic Material
Struggling with Genetics?
Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
Which of the following information cannot be determined from the base-pair sequence of a cDNA clone?
A
The amino acid sequence of the encoded protein
B
The coding sequence of the mRNA
C
The presence of untranslated regions (UTRs)
D
The intron-exon structure of the original gene
Verified step by step guidance1
Understand what a cDNA clone represents: cDNA is complementary DNA synthesized from an mRNA template, so it reflects the mature mRNA sequence without introns.
Recognize that the base-pair sequence of cDNA includes the coding sequence of the mRNA, which can be translated to determine the amino acid sequence of the encoded protein using the genetic code.
Note that cDNA also contains untranslated regions (UTRs) present in the mature mRNA, so these can be identified from the cDNA sequence.
Recall that introns are non-coding sequences present in the original genomic DNA but are spliced out during mRNA processing, so they are absent in cDNA.
Conclude that since cDNA lacks introns, the intron-exon structure of the original gene cannot be determined from the cDNA sequence alone.
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