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Multiple Choice
Which of the following best describes how cDNA synthesized in a laboratory compares to the original DNA from which it was derived?
A
cDNA is double-stranded RNA, whereas the original DNA is double-stranded DNA.
B
cDNA contains only the introns of the original DNA.
C
cDNA is identical to the original DNA, including all introns and regulatory regions.
D
cDNA contains only the exons present in the mature mRNA, while the original DNA contains both exons and introns.
Verified step by step guidance
1
Understand what cDNA (complementary DNA) is: it is synthesized from mature mRNA using the enzyme reverse transcriptase in the laboratory.
Recall that mature mRNA is processed RNA that has had its introns (non-coding regions) removed, leaving only exons (coding sequences).
Recognize that because cDNA is made from mature mRNA, it contains only the exons and lacks introns, unlike the original genomic DNA which contains both exons and introns.
Note that cDNA is double-stranded DNA, not RNA, because the reverse transcription process creates a DNA copy of the RNA template.
Conclude that cDNA differs from the original DNA in that it represents only the expressed coding sequences (exons) without introns or regulatory regions.