Open Question
What is the difference between nonhomologous end-joining (NHEJ) and homology-directed repair (HDR) in the context of genome editing?
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18. Molecular Genetic Tools
Genetic Cloning
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In genetic cloning, the primary function of DNA ligase in recombinant DNA technology is to:
A
Separate DNA fragments based on size using an electric field in agarose gel
B
Cut DNA at specific recognition sequences to create sticky or blunt ends
C
Covalently join DNA fragments by sealing nicks in the sugar-phosphate backbone, forming phosphodiester bonds
D
Synthesize complementary DNA strands using an RNA template
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