Here are the essential concepts you must grasp in order to answer the question correctly.
Gene Regulatory Sequences
Gene regulatory sequences are DNA regions that control the timing, location, and level of gene expression. In the case of even-skipped (Eve), specific enhancers respond to transcription factors to activate or repress expression in precise spatial patterns, such as the formation of stripes in Drosophila embryos.
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Transcription Factor Gradients and Combinatorial Control
Sharp expression boundaries arise from the combined effects of activator and repressor transcription factors distributed in gradients. For Eve Stripe 2, activators like Bicoid and Hunchback promote expression, while repressors like Giant and Kruppel inhibit it, creating a precise spatial pattern through their overlapping domains.
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Enhancer-Mediated Spatial Patterning
Enhancers integrate multiple transcription factor inputs to generate distinct expression domains. The Eve Stripe 2 enhancer contains binding sites for both activators and repressors, enabling it to interpret positional information and produce a sharp, well-defined stripe of gene expression in the embryo.
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