Describe the differences between genetic complementation and recombination as they relate to the detection of wild-type lysis by a mutant bacteriophage.
Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
5. Genetics of Bacteria and Viruses
Bacteriophage Genetics
Problem 14
Textbook Question
The bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?

1
Understand the context: Bacteriophages are viruses that infect bacteria. Their genomes encode proteins necessary for assembling the phage structure, but they lack the machinery required for protein synthesis, such as ribosomes.
Recall the role of the host: Since the phage genome lacks genes for ribosome structure, the bacteriophage relies on the host bacterium's ribosomes to synthesize its proteins.
Explain the process: After the phage infects the bacterial cell, it injects its genome into the host. The host's transcriptional and translational machinery (including ribosomes) is hijacked to express the phage genes.
Describe transcription and translation: The phage genes are transcribed into mRNA by the host's RNA polymerase. These mRNA molecules are then translated into proteins by the host's ribosomes, tRNAs, and other translational components.
Conclude the synthesis process: The proteins synthesized by the host's ribosomes are assembled into the structural components of the bacteriophage, such as the head, collar, tail, and tail fibers, completing the phage replication cycle.

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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Bacteriophage Structure and Function
Bacteriophages, or phages, are viruses that infect bacteria. They consist of a protein coat (capsid) that encases their genetic material, which can be either DNA or RNA. Understanding the structure of phages is crucial, as it informs how they interact with host cells and utilize the host's machinery for replication and protein synthesis.
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Mapping Bacteriophages
Transcription and Translation
Transcription is the process by which the genetic information in DNA is copied into messenger RNA (mRNA), while translation is the subsequent process where ribosomes synthesize proteins based on the mRNA sequence. In the context of phages, understanding these processes is essential to grasp how phage genes are expressed and proteins are produced despite the absence of ribosomal genes in their genomes.
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Translation initiation
Host Ribosomes and Protein Synthesis
Bacteriophages rely on the host bacterial ribosomes for protein synthesis, as they do not possess the genes necessary to produce their own ribosomal components. After infection, phage mRNA is translated by the host's ribosomes, allowing the phage to hijack the bacterial protein synthesis machinery to produce its structural proteins and enzymes needed for replication.
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