Compare methods for constructing homologous recombinant transgeni...

Question

Compare methods for constructing homologous recombinant transgenic mice and yeast.

Accepted Answer

Hi, welcome back. Let's look at the next question. Homologous recombinant transgenic mice are usually created using embryonic stem cells, es cells. Whereas homologous recombinant yeast is typically created through a process known as gene targeting. Which of the following statements best describes the difference between these processes. Choice. AES cells are isolated from developing mice embryos. While gene targeting involves introducing a linear DNA molecule into yeast cells. B gene targeting is more efficient in mice than in yeast. Ce S cells are single celled fungi. While gene targeting involves the use of embryonic stem cells or choice D homologous recombination is generally more efficient in yeast than in mice. Well, if we're talking specifically about the differences between using ES stem cells or ES cells, excuse me and gene targeting and yeast, it would be choice. A yes. Cells are isolated from developing mice embryos. All gene targeting involves introducing a linear DNA molecule into yeast cells. The ES cells have the targeted mutation created using homologous recombination and then those modified ES cells are injected into an embryo. It develops into a chimeric offspring and then the chimeras are bred until you have uh a an offspring carrying the mutation in its germline cells. And you therefore have created a transgenic line. One of the differences here is that of course mice being deployed, you have to modify two copies of the gene. Whereas yeast molecules being haploid, you only have to identify or only have to modify excuse me one chromosome. And therefore, with yeast, you can introduce linear DNA molecules or plasmids into the cells and have homologous recombination occur easily that way. So choice A is our answer. But let's look at the other answer. Choices. Choice B gene targeting is more efficient than in mice than in yeast. Uh We don't have any information that indicates this, that doesn't describe the key difference between these processes. So will cross out choice. B Choi C says ES cells are single celled fungi. Well, this is not correct. They're diploid mouse cells in this example. So that makes this incorrect. So choice C is pretty easy to eliminate. And then choice D homologous recombination is generally more efficient in yeast than in mice. Um Well, this doesn't, isn't a difference that's supported by the information homologous recombination is occurring in both cases just by two different methods. Um It's the method that's used to introduce the mutation. Then the process of getting the mutation into the organism, whether the mouse or the yeast is what's different here. So choice D is not correct. So again, the best description of the difference between using ES cells to create m transgenic mice through homologous recombination versus gene targeting in yeast is choice. A ES cells are isolated from developing mouse embryos. While gene targeting involves introducing a linear DNA molecule into yeast cells. See you in the next video.

Compare methods for constructing homologous recombinant transgenic mice and yeast.

Key Concepts

Homologous Recombination

Transgenic Organisms

Gene Targeting Techniques

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