Compare and contrast WGS to a map-based cloning approach.
How do high-throughput techniques such as computer-automated, next-generation sequencing, and mass spectrometry facilitate research in genomics and proteomics? Explain.
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Key Concepts
Next-Generation Sequencing (NGS)
Mass Spectrometry in Proteomics
High-Throughput Automation and Data Analysis
What is bioinformatics, and why is this discipline essential for studying genomes? Provide two examples of bioinformatics applications.
Annotation involves identifying genes and gene-regulatory sequences in a genome. List and describe characteristics of a genome that are hallmarks for identifying genes in an unknown sequence. What characteristics would you look for in a bacterial genome? A eukaryotic genome?
BLAST searches and related applications are essential for analyzing gene and protein sequences. Define BLAST, describe basic features of this bioinformatics tool, and give an example of information provided by a BLAST search.
Describe three major goals of the Human Genome Project.
Describe the human genome in terms of genome size, the percentage of the genome that codes for proteins, how much is composed of repetitive sequences, and how many genes it contains. Describe two other features of the human genome.
