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Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization
Sanders - Genetic Analysis: An Integrated Approach 3rd Edition
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
Chapter 10, Problem 20

A plant breeder would like to develop a seedless variety of cucumber from two existing lines. Line A is a tetraploid line, and line B is a diploid line. Describe the breeding strategy that will produce a seedless line, and support your strategy by describing the results of crosses.

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span>Step 1: Understand the ploidy levels of the parent lines. Line A is tetraploid (4n), meaning it has four sets of chromosomes, while Line B is diploid (2n), meaning it has two sets of chromosomes.
span>Step 2: Consider the cross between the tetraploid Line A (4n) and the diploid Line B (2n). This cross will produce triploid offspring (3n), as the gametes from Line A will contribute two sets of chromosomes (2n) and the gametes from Line B will contribute one set of chromosomes (n).
span>Step 3: Recognize that triploid plants (3n) are often sterile because they have an odd number of chromosome sets, which leads to problems during meiosis. This sterility is what results in seedlessness, as the plants cannot produce viable seeds.
span>Step 4: To develop a seedless cucumber variety, the breeder should focus on producing and selecting triploid plants from the cross between Line A and Line B. These triploid plants will be the seedless variety due to their inability to undergo normal meiosis.
span>Step 5: Evaluate the triploid plants for desirable traits such as fruit quality and yield, and propagate them vegetatively (e.g., through cuttings) to maintain the seedless characteristic in the new cucumber variety.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Polyploidy

Polyploidy refers to the condition of having more than two complete sets of chromosomes. In this case, Line A is tetraploid, meaning it has four sets of chromosomes. This genetic makeup can lead to increased size and vigor in plants, and is often utilized in breeding programs to create new varieties with desirable traits, such as seedlessness.
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Autopolyploidy

Diploidy

Diploidy is the state of having two complete sets of chromosomes, which is the typical condition for most plants, including Line B in this scenario. When breeding diploid and polyploid plants, the resulting offspring can exhibit unique genetic combinations, which can be manipulated to achieve specific traits, such as seedlessness in cucumbers.
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Diploid Genetics

Hybridization and Backcrossing

Hybridization involves crossing two different lines to produce offspring with combined traits. In this breeding strategy, the tetraploid Line A can be crossed with the diploid Line B to create triploid offspring, which are often sterile and thus seedless. Backcrossing these triploids with one of the parent lines can help stabilize the desired seedless trait in subsequent generations.
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Related Practice
Textbook Question

Histone protein H4 isolated from pea plants and cow thymus glands contains 102 amino acids in both cases. A total of 100 of the amino acids are identical between the two species. Give an evolutionary explanation for this strong amino acid sequence identity based on what you know about the functions of histones and nucleosomes.

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Textbook Question

A survey of organisms living deep in the ocean reveals two new species whose DNA is isolated for analysis. DNA samples from both species are treated to remove nonhistone proteins. Each DNA sample is then treated with DNase I that cuts DNA not protected by histone proteins but is unable to cut DNA bound by histone proteins. Following DNase I treatment, DNA samples are subjected to gel electrophoresis, and the gels are stained to visualize all DNA bands in the gel. The staining patterns of DNA bands from each species are shown in the figure. The number of base pairs in small DNA fragments is shown at the left of the gel. Interpret the gel results in terms of chromatin organization and the spacing of nucleosomes in the chromatin of each species.

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Textbook Question

In humans that are XX/XO mosaics, the phenotype is highly variable, ranging from females who have classic Turner syndrome symptoms to females who are essentially normal. Likewise, XY/XO mosaics have phenotypes that range from Turner syndrome females to essentially normal males. How can the wide range of phenotypes be explained for these sex-chromosome mosaics?

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Textbook Question

In Drosophila, seven partial deletions (1 to 7) shown as gaps in the following diagram have been mapped on a chromosome. This region of the chromosome contains genes that express seven recessive mutant phenotypes, identified in the following table as a through g. A researcher wants to determine the location and order of genes on the chromosome, so he sets up a series of crosses in which flies homozygous for a mutant allele are crossed with flies homozygous for a partial deletion. The progeny are scored to determine whether they have the mutant phenotype ('m' in the table) or the wild-type phenotype ('+' in the table). Use the partial deletion map and the table of progeny phenotypes to determine the order of genes on the chromosome.

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Textbook Question

Two experimental varieties of strawberry are produced by crossing a hexaploid line that contains 48 chromosomes and a tetraploid line that contains 32 chromosomes. Experimental variety 1 contains 40 chromosomes, and experimental variety 2 contains 56 chromosomes.

Do you expect both experimental lines to be fertile? Why or why not?

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Textbook Question

Two experimental varieties of strawberry are produced by crossing a hexaploid line that contains 48 chromosomes and a tetraploid line that contains 32 chromosomes. Experimental variety 1 contains 40 chromosomes, and experimental variety 2 contains 56 chromosomes.

How many chromosomes from the hexaploid line are contributed to experimental variety 1? To experimental variety 2?

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