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Ch. 10 - Eukaryotic Chromosome Abnormalities and Molecular Organization
Sanders - Genetic Analysis: An Integrated Approach 3rd Edition
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172Not the one you use?Change textbook
Chapter 10, Problem 17

Histone protein H4 isolated from pea plants and cow thymus glands contains 102 amino acids in both cases. A total of 100 of the amino acids are identical between the two species. Give an evolutionary explanation for this strong amino acid sequence identity based on what you know about the functions of histones and nucleosomes.

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Understand the role of histones: Histones are proteins that help package DNA into nucleosomes, which are the fundamental units of chromatin structure. This packaging is essential for DNA stability, regulation of gene expression, and proper chromosome segregation during cell division.
Recognize the evolutionary importance of histone H4: Histone H4 is one of the core histones involved in forming nucleosomes. Its function is highly conserved across species because it plays a critical role in maintaining the structural integrity of chromatin and ensuring proper cellular function.
Consider the consequences of mutations: Mutations in histone H4 could disrupt chromatin structure, leading to issues with DNA replication, repair, and gene expression. Such disruptions could be detrimental to the organism's survival, explaining why the amino acid sequence is highly conserved.
Analyze the sequence conservation: The fact that 100 out of 102 amino acids are identical between pea plants and cow thymus glands suggests strong evolutionary pressure to maintain the sequence. This indicates that these amino acids are critical for the protein's function and cannot be easily altered without negative effects.
Conclude with the evolutionary explanation: The strong amino acid sequence identity between species reflects the essential and universal role of histone H4 in chromatin structure and function. Evolution has preserved this sequence to ensure the survival and proper functioning of diverse organisms.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Histone Proteins

Histone proteins are essential components of chromatin, which package and order DNA into structural units called nucleosomes. They play a critical role in gene regulation and DNA accessibility, influencing how genes are expressed. The conservation of histone proteins across species suggests their fundamental importance in maintaining cellular functions and stability.
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Amino Acid Sequence Conservation

Amino acid sequence conservation refers to the phenomenon where certain sequences of amino acids remain unchanged across different species due to their essential roles in protein function. In the case of histone H4, the high degree of identity (100 out of 102 amino acids) indicates that these specific residues are crucial for the protein's structural integrity and its interaction with DNA, which has been preserved through evolution.
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Sequencing Overview

Evolutionary Pressure and Function

Evolutionary pressure drives the conservation of certain traits or sequences that are vital for survival and reproduction. In histones, the strong conservation of amino acids suggests that any significant changes could disrupt their function, leading to detrimental effects on gene regulation and chromatin structure. This evolutionary stability highlights the importance of histones in cellular processes across diverse organisms.
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Functional Genomics
Related Practice
Textbook Question

The accompanying chromosome diagram represents a eukaryotic chromosome prepared with Giemsa stain. Indicate the heterochromatic and euchromatic regions of the chromosome, and label the chromosome's centromeric and telomeric regions.

Do you expect the centromeric region to contain heterochromatin? Why or why not?

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Textbook Question

The accompanying chromosome diagram represents a eukaryotic chromosome prepared with Giemsa stain. Indicate the heterochromatic and euchromatic regions of the chromosome, and label the chromosome's centromeric and telomeric regions.

Why are expressed genes not found in the telomeric region of chromosomes?

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Textbook Question

The accompanying chromosome diagram represents a eukaryotic chromosome prepared with Giemsa stain. Indicate the heterochromatic and euchromatic regions of the chromosome, and label the chromosome's centromeric and telomeric regions.

Are you more likely to find the DNA sequence encoding the digestive enzyme amylase in a heterochromatic, euchromatic, centromeric, or telomeric region? Explain your reasoning.

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Textbook Question

A survey of organisms living deep in the ocean reveals two new species whose DNA is isolated for analysis. DNA samples from both species are treated to remove nonhistone proteins. Each DNA sample is then treated with DNase I that cuts DNA not protected by histone proteins but is unable to cut DNA bound by histone proteins. Following DNase I treatment, DNA samples are subjected to gel electrophoresis, and the gels are stained to visualize all DNA bands in the gel. The staining patterns of DNA bands from each species are shown in the figure. The number of base pairs in small DNA fragments is shown at the left of the gel. Interpret the gel results in terms of chromatin organization and the spacing of nucleosomes in the chromatin of each species.

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Textbook Question

In humans that are XX/XO mosaics, the phenotype is highly variable, ranging from females who have classic Turner syndrome symptoms to females who are essentially normal. Likewise, XY/XO mosaics have phenotypes that range from Turner syndrome females to essentially normal males. How can the wide range of phenotypes be explained for these sex-chromosome mosaics?

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Textbook Question

A plant breeder would like to develop a seedless variety of cucumber from two existing lines. Line A is a tetraploid line, and line B is a diploid line. Describe the breeding strategy that will produce a seedless line, and support your strategy by describing the results of crosses.

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