Here are the essential concepts you must grasp in order to answer the question correctly.
DNA Polymerase
DNA polymerase is an essential enzyme that synthesizes new DNA strands by adding nucleotides complementary to the template strand during DNA replication. It plays a critical role in ensuring the accuracy and fidelity of DNA replication, which is vital for cell division and genetic inheritance.
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Restriction Enzymes
Restriction enzymes, also known as restriction endonucleases, are proteins that cut DNA at specific sequences. They are widely used in molecular biology for cloning, DNA analysis, and genetic engineering, allowing scientists to manipulate DNA fragments for various applications.
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Ligase
DNA ligase is an enzyme that facilitates the joining of DNA strands together by forming phosphodiester bonds. It is crucial in processes such as DNA replication and repair, as it seals nicks in the sugar-phosphate backbone, ensuring the integrity of the DNA molecule.
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