Textbook Question
Which application of recombinant DNA technology involves replacing a nonfunctional, mutated gene with a functional gene?
a. Gene therapy
b. Functional genomics
c. Genetic screening
d. Protein synthesis
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17. Biotechnology
Introduction to DNA-Based Technology
1:30 minutesProblem 7
Textbook Question
Pieces of human DNA stored in yeast cells.
a. Antisense
b. Clone
c. Library
d. Southern blot
e. Vector
Verified step by step guidance1
Understand the key terms provided: antisense, clone, library, Southern blot, and vector, as they relate to molecular biology and microbiology techniques.
Recall that a 'library' in molecular biology refers to a collection of DNA fragments stored in host cells, such as bacteria or yeast, representing the genome or a subset of DNA sequences.
Recognize that 'pieces of human DNA stored in yeast cells' describes a DNA library maintained within yeast, which is a common host for cloning large DNA fragments.
Match the description 'Pieces of human DNA stored in yeast cells' to the term 'library' because it best fits the concept of a collection of DNA fragments stored in a host organism.
Confirm that other options like 'clone' refer to identical copies of DNA, 'vector' is a DNA molecule used to carry foreign DNA into a host, 'antisense' relates to RNA sequences complementary to mRNA, and 'Southern blot' is a technique for detecting specific DNA sequences.
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Video duration:
1mKey Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Clone
A clone refers to a population of cells or DNA molecules derived from a single ancestor, containing identical genetic material. In molecular biology, cloning involves creating copies of specific DNA fragments, often by inserting them into host cells like yeast or bacteria for replication.
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Vector
A vector is a DNA molecule used as a vehicle to transfer foreign genetic material into a host cell. Common vectors include plasmids and viruses, which facilitate the replication and expression of inserted DNA fragments within host organisms such as yeast.
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Library
A DNA library is a collection of cloned DNA fragments that represent the entire genome or a subset of an organism's DNA. These fragments are stored within host cells, like yeast, allowing researchers to access and study specific genes or sequences.
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