Textbook Question
Self-replicating DNA for transmitting a gene from one organism to another.
a. Antisense
b. Clone
c. Library
d. Southern blot
e. Vector
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Ch. 9 - Biotechnology & DNA Technology
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398
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Table of contents
- Ch. 1 - The Microbial World and You18
- Ch. 2 - Chemical Principles20
- Ch. 3 - Observing Microorganisms Through a Microscope18
- Ch. 4 - Functional Anatomy of Prokaryotic and Eukaryotic Cells21
- Ch. 5 - Microbial Metabolism20
- Ch. 6 - Microbial Growth20
- Ch. 7 - The Control of Microbial Growth20
- Ch. 8 - Microbial Genetics19
- Ch. 9 - Biotechnology & DNA Technology20
- Ch. 10 - Classification of Microorganisms15
- Ch. 11 - The Prokaryotes: Domains Bacteria and Archaea14
- Ch. 12 - The Eukaryotes: Fungi, Algae, Protozoa, and Helminths17
- Ch. 13 - Viruses, Viroids, and Prions20
- Ch. 14 - Principles of Disease and Epidemiology21
- Ch. 15 - Microbial Mechanisms of Pathogenicity19
- Ch. 16 - Innate Immunity: Nonspecific Defenses of the Host20
- Ch. 17 - Adaptive Immunity: Specific Defenses of the Host20
- Ch. 18 - Practical Applications of Immunology20
- Ch. 19 - Disorders Associated with the Immune System19
- Ch. 20 - Antimicrobial Drugs19
- Ch. 21 - Microbial Diseases of the Skin and Eyes21
- Ch. 22 - Microbial Diseases of the Nervous System19
- Ch. 23 - Microbial Diseases of the Cardiovascular and Lymphatic Systems20
- Ch. 24 - Microbial Diseases of the Respiratory System20
- Ch. 25 - Microbial Diseases of the Digestive System20
- Ch. 26 - Microbial Diseases of the Urinary and Reproductive Systems19
- Ch. 27 - Environmental Microbiology20
- Ch. 28 - Applied and Industrial Microbiology19
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Not the one you use?Change textbook
Chapter 9, Problem 8
A population of cells carrying a desired plasmid.
a. Antisense
b. Clone
c. Library
d. Southern blot
e. Vector
Verified step by step guidance1
Understand the key terms provided: 'antisense', 'clone', 'library', 'Southern blot', and 'vector'. Each term has a specific meaning in molecular biology and microbiology.
Focus on the phrase 'A population of cells carrying a desired plasmid.' This implies multiple cells that all contain the same plasmid, which is a circular DNA molecule used in cloning.
Recall that a 'clone' in microbiology refers to a group of genetically identical cells derived from a single ancestor cell, often carrying the same plasmid.
Match the statement to the term that best fits the description of identical cells carrying the same plasmid, which is 'clone'.
Confirm that other options do not fit: 'antisense' relates to RNA sequences, 'library' is a collection of DNA fragments, 'Southern blot' is a DNA detection method, and 'vector' is the DNA molecule used to carry foreign DNA but not the population of cells itself.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Clone
A clone refers to a population of genetically identical cells derived from a single ancestor cell, often carrying the same plasmid or DNA sequence. In molecular biology, cloning is used to amplify a specific DNA fragment within host cells.
Recommended video:
Guided course
02:25
Introduction to DNA Cloning
Plasmid
A plasmid is a small, circular DNA molecule independent of chromosomal DNA, commonly found in bacteria. It can replicate autonomously and is often used as a vector to carry foreign genes into host cells for cloning or expression.
Recommended video:
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05:57F Plasmids
Vector
A vector is a DNA molecule, such as a plasmid or virus, used to transfer genetic material into a host cell. Vectors contain necessary elements for replication and selection, enabling the propagation and expression of inserted genes.
Recommended video:
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03:41Cloning with Recombinant DNA
Related Practice
Textbook Question
Pieces of human DNA stored in yeast cells.
a. Antisense
b. Clone
c. Library
d. Southern blot
e. Vector
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Textbook Question
Describe an rDNA experiment in two or three sentences. Use the following terms: intron, exon, DNA, mRNA, cDNA, RNA polymerase, reverse transcriptase.
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Textbook Question
You are attempting to insert a gene for saltwater tolerance into a plant by using the Ti plasmid. In addition to the desired gene, you add a gene for tetracycline resistance (tet) to the plasmid. What is the purpose of the tet gene?
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Textbook Question
List at least two examples of the use of rDNA in medicine and in agriculture.
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Textbook Question
How does RNAi “silence” a gene?
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