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Multiple Choice
A particular triplet of bases in the template strand of DNA is 5′-AGT-3′. What would be the corresponding codon for the mRNA that is transcribed?
A
3′-UCA-5′.
B
3′-UGA-5′.
C
5′-TCA-3′.
D
3′-ACU-5′.
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Verified step by step guidance
1
Understand that DNA transcription involves creating a complementary RNA strand from the DNA template strand. The template strand is read in the 3′ to 5′ direction to synthesize the mRNA in the 5′ to 3′ direction.
Identify the given DNA template strand sequence: 5′-AGT-3′. Since transcription reads the template strand in the 3′ to 5′ direction, reverse the sequence to 3′-TGA-5′.
Determine the complementary RNA bases for each DNA base: Adenine (A) pairs with Uracil (U), Thymine (T) pairs with Adenine (A), and Guanine (G) pairs with Cytosine (C).
Apply the base pairing rules to the reversed DNA sequence (3′-TGA-5′) to find the mRNA sequence: T pairs with A, G pairs with C, and A pairs with U, resulting in the mRNA sequence 5′-ACU-3′.
Reverse the mRNA sequence to match the typical representation of mRNA codons, which are written in the 5′ to 3′ direction: 3′-ACU-5′.