Which sequence correctly summarizes the standard broth dilution method for determining MIC?

Mix equal amounts of antibiotic and bacterial suspension on agar, let the mixture dry, then use turbidity measured spectrophotometrically to determine the lowest antibiotic concentration that causes agar clearing.

Prepare serial dilutions of antibiotic in liquid growth medium, inoculate each tube with a standardized bacterial inoculum, incubate, and read MIC as the lowest concentration with no visible turbidity.

Apply antibiotic discs to a liquid broth surface and measure the diameter of broth clearing around each disc to determine MIC, with the largest clearing corresponding to the lowest MIC value.

Use broth with no bacteria, incubate with serially increasing amounts of antibiotic and measure pH changes to infer bacterial susceptibility, with stable pH indicating inhibition and therefore MIC.