Question 1

Which part of the microscope is responsible for focusing the specimen by making large adjustments to the stage height?

Accepted Answer

Coarse adjustment knob

Question 2

What is the function of the condenser in a compound light microscope?

Accepted Answer

It focuses light onto the specimen.

Question 3

If you are using a 10× ocular lens and a 40× objective lens, what is the total magnification? Use the formula $$	ext{Total magnification} = 	ext{ocular} 	imes 	ext{objective}.$$

Accepted Answer

$$10 	imes 40 = 400$$

Question 4

Which part of the microscope should you use to make small, precise focusing adjustments?

Accepted Answer

Fine adjustment knob

Question 5

What is the main purpose of the oil used in oil immersion microscopy?

Accepted Answer

To reduce light refraction and improve resolution

Question 6

Which part of the microscope is used to change the objective lenses?

Accepted Answer

Nose piece

Question 7

What is the function of the diaphragm on a microscope?

Accepted Answer

To adjust the amount of light passing through the specimen

Question 8

Which part of the microscope supports the entire instrument and provides stability?

Accepted Answer

Base

Question 9

If the condenser is not properly aligned, what is the most likely effect on your image?

Accepted Answer

The image will be blurry or lack contrast

Question 10

Which part of the microscope is used to move the slide smoothly in the X and Y directions for scanning the specimen?

Accepted Answer

Stage control knobs

Question 11

What is the function of the ocular lens (eyepiece) in a microscope?

Accepted Answer

It provides the initial magnification of the specimen

Question 12

Which part of the microscope should you hold when carrying it to ensure safety?

Accepted Answer

Arm (carrying handle)

Question 13

What is the main function of the stage on a microscope?

Accepted Answer

To support the slide being observed

Question 14

Which part of the microscope is responsible for illuminating the specimen?

Accepted Answer

Illuminator (light source)

Question 15

If you want to increase the resolution of your image, which adjustment should you make?

Accepted Answer

Use oil immersion with the 100× objective lens

Microbiology Study Guide: Foundations, Microscopy, and Lab Techniques

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