Ch. 9 - Biotechnology & DNA Technology

Tortora - Microbiology: An Introduction 14th Edition

Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398Not the one you use?Change textbook

Tortora 14th Edition

Ch. 9 - Biotechnology & DNA Technology

Problem 9

Chapter 9, Problem 9

Self-replicating DNA for transmitting a gene from one organism to another.
a. Antisense
b. Clone
c. Library
d. Southern blot
e. Vector

Verified step by step guidance

Identify the key concept in the statement: 'Self-replicating DNA for transmitting a gene from one organism to another.' This refers to a DNA molecule that can replicate independently and is used to carry genetic material between organisms.

Recall the definitions of the given options:

a. antisense - a strand of nucleic acid complementary to a messenger RNA (mRNA) strand, used to block gene expression.

b. clone - a genetically identical copy of a DNA fragment or organism.

c. library - a collection of DNA fragments cloned into vectors, representing the genome or transcriptome of an organism.

d. Southern blot - a laboratory method used to detect specific DNA sequences in DNA samples.

e. vector - a DNA molecule used as a vehicle to transfer foreign genetic material into another cell, typically self-replicating.

Match the statement to the correct term: since the statement describes self-replicating DNA used to transmit genes, the correct match is the term that defines such a DNA molecule, which is a 'vector'.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Vector

A vector is a self-replicating DNA molecule used to transfer genetic material between organisms. Common vectors include plasmids and viruses, which facilitate gene cloning and expression in host cells.

Cloning

Cloning involves creating identical copies of a DNA fragment or organism. In molecular biology, it refers to inserting a DNA fragment into a vector to produce multiple copies within a host.

Gene Library

A gene library is a collection of DNA fragments cloned into vectors, representing the entire genome or specific genes of an organism. It allows researchers to isolate and study individual genes.

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Related Practice

Textbook Question

A population of cells carrying a desired plasmid.

a. Antisense

b. Clone

c. Library

d. Southern blot

e. Vector

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Textbook Question

You are attempting to insert a gene for saltwater tolerance into a plant by using the Ti plasmid. In addition to the desired gene, you add a gene for tetracycline resistance (tet) to the plasmid. What is the purpose of the tet gene?

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Textbook Question

Match the following choices to the statement provided below.

a. Antisense

b. Clone

c. Library

d. Southern blot

e. Vector

DNA that hybridizes with mRNA.

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