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Multiple Choice
Which of the following is not used in the endospore staining procedure?
A
Crystal violet
B
Malachite green
C
Safranin
D
Heat
Verified step by step guidance
1
Understand the purpose of endospore staining: It is used to identify bacterial endospores, which are resistant structures formed by some bacteria.
Identify the common reagents used in endospore staining: Typically, malachite green is used as the primary stain, and safranin is used as a counterstain.
Recognize the role of heat in the procedure: Heat is applied to help the primary stain penetrate the endospore, which is resistant to staining.
Consider the role of crystal violet: Crystal violet is commonly used in Gram staining, not in endospore staining.
Conclude which reagent is not used in endospore staining: Based on the typical procedure, crystal violet is not used in the endospore staining process.