Here are the essential concepts you must grasp in order to answer the question correctly.
Glycosidic Linkages
Glycosidic linkages are covalent bonds that connect monosaccharides to form polysaccharides. They can be classified as α or β based on the orientation of the hydroxyl group on the first carbon of the glucose monomer. Amylase specifically targets α-glycosidic linkages, which are found in starch and glycogen, allowing it to break these down into simpler sugars.
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Formation & Breakdown of Polysaccharides
Types of Polysaccharides
Polysaccharides are large carbohydrate molecules composed of long chains of monosaccharide units. Common examples include starch, glycogen, cellulose, and chitin. Starch and glycogen contain α-glycosidic linkages, making them substrates for amylase, while cellulose and chitin contain β-glycosidic linkages, which amylase cannot break down.
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Formation & Breakdown of Polysaccharides
Enzyme Specificity
Enzyme specificity refers to the ability of an enzyme to select and catalyze a particular substrate among many possible molecules. Amylase is specific for α-glycosidic linkages, meaning it will only act on polysaccharides like starch and glycogen, but not on those with β-glycosidic linkages, such as cellulose and chitin. This specificity is crucial for understanding which substrates can be broken down by amylase.
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