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Multiple Choice
In DNA replication, what enzyme unwinds or “unzips” the parental double-stranded DNA at the replication fork?
A
DNA polymerase III
B
DNA helicase
C
DNA ligase
D
Primase
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Verified step by step guidance
1
Understand the role of each enzyme listed in the context of DNA replication.
Recall that DNA polymerase III is primarily responsible for synthesizing the new DNA strand by adding nucleotides complementary to the template strand.
Recognize that DNA ligase functions to join Okazaki fragments on the lagging strand by forming phosphodiester bonds, sealing nicks in the sugar-phosphate backbone.
Know that primase synthesizes short RNA primers needed to initiate DNA synthesis by DNA polymerase.
Identify that DNA helicase is the enzyme responsible for unwinding or "unzipping" the parental double-stranded DNA at the replication fork by breaking hydrogen bonds between base pairs, allowing replication to proceed.